Background: Safeguarding the integrity of steatotic livers during transplantation is paramount to minimize the impact of ischemia-reperfusion injury. This investigation scrutinizes the intricate biochemical makeup of perfusates obtained from Zucker obese rat livers following a 24-hour stint of cold storage at 4°C. Various preservation solutions are employed, including University of Wisconsin (UW), Histidine-Tryptophan-Ketoglutarate (HTK), IGL-2, and a tailored version of IGL-2: IGL-2Modified (IGL-2M) to be more stable at room temperature.
Methods: Male Zucker obese rat livers undergo a meticulous 24-hour cold preservation at 4°C utilizing UW, HTK, IGL-2, and IGL-2M. A control SHAM group undergoes identical procedures without preservation. The subsequent generation of perfusates for analysis involves a thorough Ringer's solution washout. Tissue samples are also obtained. Key parameters encompass ALT, AST, GLDH, lactate, nitrites and nitrates, uric acid, syndecan, succinate, pH and glucose.
Results: Preservation with HTK results in a substantial elevation of transaminases (ALT, AST), GLDH, and uric acid levels when compared to alternative solutions. In contrast to IGL-2, IGL-2M exhibits a conspicuous surge in nitrites and nitrates, delineating its distinct biochemical profile. Employment of UW leads to heightened syndecan levels, potentially associated with an augmented shedding of glycocalyx. Both IGL-2 and IGL-2M demonstrate analogous succinate release levels, underscoring the comparable outcomes between these two formulations.
Conclusions: The identified markers in this study emerge as valuable tools for evaluating the status of the liver through perfusate analysis reflecting liver status. IGL-2 and IGL-2M exhibit promising outcomes, characterized by a diminished presence of oxidative stress and an augmented preservation of specific biochemical markers. The imperative for further validation and exploration of these markers persists, as they could significantly contribute to the formulation of effective strategies for assessing steatotic liver conditions during transplantation.