Abstract Introduction: Kidney transplantation from living donors has revolutionized treatment for end-stage renal disease. It is not only a method to increase access to kidney transplantation but can also offer recipients significantly improved outcomes compared to deceased donor transplants. This improvement is marked by better graft survival rates, reduced waiting times, and enhanced quality of life post-transplantation. In Libya, it is the only way to help those in need of a transplant since Deceased Organ Donation “DOD” has not started yet. In Libya, the demand for kidney transplants has steadily increased, mirroring global trends. However, this growing demand is challenged by a limited supply of eligible donors, exacerbated by strict eligibility criteria necessary to ensure donor and recipient safety. Objective: To comprehensively investigate and categorize the primary reasons for potential donor ineligibility in living donor kidney transplants at the Libyan transplant Authority. Methods: A retrospective cohort study was conducted, analyzing medical records from 2004 to 2023 at the Libyan transplant Authority. Criteria for potential donor ineligibility were identified and categorized. Results: The most common causes of ineligibility were ABO incompatibility, immunological, anatomical, medical, psychological, and social factors. A significant correlation was found between donor ineligibility and older age. Conclusion: Understanding the reasons for potential donor ineligibility can enhance pre-screening processes and donor counseling, potentially increasing the donor pool.