Islet transplantation is one of the most popular studies in the treatment of type 1 diabetes. Early islet apoptosis is a key problem affecting the efficacy of islet transplantation and its wide clinical application. Extensive studies have shown that combined transplantation of bone marrow mesenchymal stem cells(BMSCs)and islet cells is the main strategy to inhibit the apoptosis of grafted islets. At the same time, studies have shown that pretreated mesenchymal stem cells show more powerful ability to reduce ischemia reperfusion injury and immunoregulation. In order to improve the transplantation efficiency and reduce the apoptosis of grafted islets，in this study, BMSCs had been pre-treated with staurosporine (BMSCs-STS) .And we successfully constructed apoptotic BMSCs. It was found that pretreatment of INS-1 cell line and primary islet with BMSCs-STS CM can inhibit the apoptosis of them. Moreover, the combination transplantation of BMSCs-STS and islet cells can reduce the blood glucose of diabetic rats to normal much earlier. Further, we used proteomics technology to search for differentially expressed proteins in BMSCs-STS CM, screened out the most likely proteins to inhibit apoptosis, and figured out the mechanism of this protein to inhibit early apoptosis of grafted islets, providing a new theoretical basis for improving the transplantation effect.